 |
|||||||||
 |
Year 2021 Volume 19, Issue 2 |
|
|||||||
|
|||||||||
Issues/2021/vol. 19 /Issue 2 |
M. D. Kucheruk, D. A. Zasiekin, R. O. Dymko
«Comprehensive Determination of the Toxicity of Disinfectant Developed on the Base of the Composition of the Nanosolution of Silver and Lactic Acid»
0433–0443 (2021)
PACS numbers: 81.16.Fg, 83.80.Lz, 87.18.Hf, 87.19.xb, 87.85.Rs
The article presents the results of the study of acute toxicity, cumulative, irritating, sensitizing, skin-resorptive actions of disinfectant—a composition of nanoparticles of silver and lactic acid on the body of laboratory animals and its effects on organs and tissues of white mice. Determination of the average lethal dose of the investigated disinfectant is performed according to the method of G. Kerber adapted and recommended for chemicals according to a special formula. Experimental and control animals are observed during the cumulative study. No animals died. At the end of experiment, euthanasia and dissection of killed mice, macroscopic changes in the internal organs are not found. When studying the irritating effect of the tool, it is found that, during the experimental period (30 days), application of the 0.5, 1.0 and 2.5% solutions do not cause any visible changes on the skin surface and changes in the physiological functions of the experimental animals. During the study of sensitizing effect, it is found that, in the case of application of all tested concentrations (100, 75 and 50%) to the skin of animals, irritant effect is not observed. A study of the presence of sensitizing properties shows that, after intradermal injection into the ear of ants, at the injection site, no skin changes are detected. Applications of the drug in a sensitizing concentration for 7 days have not an irritating effect on the skin of ants. Throughout the experiment, the skin is clean, normal colour. During studies, to determine the skin-resorptive effect of the drug, we found no signs of toxic effects of the 2.5% and 5.0% solutions in white mice. It is also experimentally proven that the studied disinfectant, when applied orally in a volume of 1 cm3 in 0.5% concentration, does not cause the visible pathological and anatomical changes. As found, in experimental animals, with oral administration of the 0.5% concentration of disinfectant, there are no histological disorders in organs and tissues. Thus, as established, the studied agent based on the composition of the silver nanoparticles and lactic acid, according to the classification of substances for toxicity (GOST 12.1.007-76), belongs to the 4th class of toxicity and has no pronounced cumulative, irritating, sensitizing and skin-resorptive actions.
Keywords: disinfectant, silver nanoparticles, lactic acid, toxicity, laboratory animals
https://doi.org/10.15407/nnn.19.02.433
References
1. M. D. Kucheruk, D. A. Zasiekin, and R. O. Dymko, Nanosistemi, Nanomateriali, Nanotehnologii, 17, No. 4: 609 (2019) (in Ukrainian); https://doi.org/10.15407/nnn.17.04.6092. A. I. Bondarchuk, V. L. Kovalenko, A. I. Chekhun et al., Veterynarna Biotekhnologiya, 24: 41 (2014) (in Ukrainian).
3. M. K. Pototskyi, Morfofunktsionalni Doslidzhennya v Normi i Patologii: Metodychni Vkazivky (Kyiv: Vydavnytstvo NAU: 2007) (in Ukrainian).
4. A. V. Hnatenko, Veterynarna Biotekhnologiya, 22: 74 (2013) (in Ukrainian).
5. V. L. Kovalenko, Metody Kontrolyu Dezinfikuyuchykh Zasobiv: Dovidnyk (Kyiv: VSP ‘IPO KNUBA’: 2014) (in Ukrainian).
6. A. I. Zavhorodniy, B. T. Stehniy, A. P. Paliy et al., Naukovi ta Praktychni Aspekty Dezinfektsii u Veterynarniy Medytsyni (Kharkiv: FOP ‘Brovin O.V.’: 2013) (in Ukrainian).
7. I. Ya. Kotsiumbas, O. H. Malyk, I. P. Patereha et al., Doklinichni Doslidzhennya Veterynarnykh Likars’kykh Zasobiv (Lviv: Triada Plyus:2006) (in Ukrainian).
8. O. M. Yakubchak, S. V. Midyk, Ya. K. Serdyukov et al., Veterynarna Medytsyna Ukrainy, 1: 28 (2006) (in Ukrainian).
9. V. L. Kovalenko and V. V. Nedosiekov, Metodychni Pidkhody Kontrolyu Dezinfikuyuchykh Zasobiv dlya Veterynarnoyi Medytsyny (Kyiv: 2011) (in Ukrainian).
10. Yu. I. Afanasiev, N. A. Yurina, E. F. Kotovskiy et al., Gistologiya, Tsitologiya i Ehmbriologiya (Moscow: Meditsina: 2002) (in Russian).
11. M. E. Dzerzhynskyi, H. V. Ostrovska, N. V. Skrypnyk, S. M. Harmatina, Histolohiia. Praktykum: Navchalnyy Posibnyk (Kyiv: Vydavnycho Poligrafichnyy Tsentr ‘Kyivskyy Universytet’: 2012) (in Ukrainian).
 This article is licensed under the Creative Commons Attribution-NoDerivatives 4.0 International License ©2003—2021 NANOSISTEMI, NANOMATERIALI, NANOTEHNOLOGII G. V. Kurdyumov Institute for Metal Physics of the National Academy of Sciences of Ukraine. E-mail: tatar@imp.kiev.ua Phones and address of the editorial office About the collection User agreement |