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Year 2019 Volume 17, Issue 4 |
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Issues/2019/vol. 17 /Issue 4 |
M. D. Kucheruk, D. A. Zasekin, R. O. Dymko
«Application of a Composition of Nanosolutions of Silver and Lactic Acid for Veterinary Disinfection»
609–619 (2019)
PACS numbers: 81.16.Pr, 83.80.Lz, 87.18.Hf, 87.19.xb, 87.85.Rs
The results of the study in vitro of antibacterial activity of disinfectant based on the silver nanoparticles and lactic acid with respect to Åscherichia coli (strain 1257) and Staphylococcus aureus (strain P-209) are presented. In veterinary practice today practically, there are no effective, environmentally friendly, and safe disinfectants. Alternative to the most commonly used active substances of modern disinfectants can be the nanoparticles of metals, which have antimicrobial properties. This study is carried out in accordance with generally accepted methods and ‘Recommendations for Sanitary-Microbiological Study of Flushing from Surfaces of Test Objects and Objects of Veterinary Supervision and Control’. The instrument is tested in concentrations of 0.05%, 0.5%, 1%, 2% and in undiluted state at exposure of 10, 30 and 60 min. In the presence of E. coli growth, the colour of the medium KODA from green colour changes to yellow one. Only these changes are observed in the control study. In none of the studied exposures and concentrations of the agent, the growth of the E. coli is not observed, since the environment does not change its colour (remaining green one). This indicates that the disinfectant disinfects the surface of the test object. The growth of Staphylococcus aureus is not observed at all the study exposures and concentrations. In the control experiment, with turbidity of the salt meal–peptone broth to confirm the growth of S. aureus, the rinses are transferred to milk–salt agar and placed in a thermostat at \(37^{\circ}C\) for 24 hours. There is an intense growth of white-yellow viscous colonies of moderate size. Thus, it is proved that, under in vitro conditions, there is no growth of Staphylococcus aureus and E. coli, due to the action of disinfectant based on the silver nanoparticles and lactic acid. The investigated disinfectant exhibits an effective bactericidal action against E. coli and Staphylococcus aureus. The smallest concentration of disinfectant studied and the exposure, at which the strains of microorganisms are died, are 0.5% at 30 min.
Keywords: disinfectant, silver nanoparticles, antimicrobial activity, golden staphylococcus, E. coli
https://doi.org/10.15407/nnn.17.04.609
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 This article is licensed under the Creative Commons Attribution-NoDerivatives 4.0 International License © NANOSISTEMI, NANOMATERIALI, NANOTEHNOLOGII G. V. Kurdyumov Institute for Metal Physics of the National Academy of Sciences of Ukraine, 2019 E-mail: tatar@imp.kiev.ua Phones and address of the editorial office About the collection User agreement |