Collection of scientific works Nanosystems, nanomaterials, nanotechnologies Year 2023 Volume 21, Issue 3
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S. M. DYBKOVA, L. S. REZNICHENKO, Z. R. ULBERG, V. I. PODOLSKA, T. G. GRUZINA, O. B. LYUTKO, K. B. VITRAK, and N. I. GRYSHCHENKO
Antibacterial Properties of Nanobiocomposite Materials Based on Biogenic Silver Nanoparticles
643–663 (2023)

PACS numbers: 81.07.Pr,81.16.Fg,87.16.-b,87.19.xb,87.64.-t,87.85.jj,87.85.Rs

The article presents the experimental results on the antibacterial properties of nanobiocomposite (NBC) material, which contains ultradispersed silver nanoparticles (NPs) synthesized with the probiotic Lactobacillus plantarum. Synthesized NBC samples are investigated with transmission and scanning electron microscopies, spectroscopy analysis and electrophoresis method. As shown, the physical and chemical properties of the NBC surface, which are determined by the silver-NPs’ concentration and the pH of the medium, play a significant role in the NBC antimicrobial activity. The electrosurface properties of the nanobiocomposite based on L. plantarum and stabilized in the matrix cell ultradispersed silver are characterized by a low surface charge and a hydrophilic surface. The electrokinetic potential correlates with the content of silver NPs. The Ag-NPs’ concentration of 15–25 mg/g is optimal and corresponds to the least damage of the cell matrix and the formation of silver NPs in a narrow size range of 2–6 nm. The maximum values of the NBC sigma-potential are in the range of pH 6.5–7.0. Such parameters ensure a high antibacterial effect of NBC. The method of determining the respiratory activity (RA) of the test strain of bacteria E. coli K–A shows the concentration-dependent sensitivity of RA to NBC. The inhibiting concentration of RA by nanobiocomposite is of 6–8 µg/ml. The antibacterial activity of NBC against 17 strains of the pathogenic and opportunistic microorganisms, which are threatening agents of nosocomial diseases and complications in surgical practice, is studied. Almost all tested strains show a wide level of sensitivity to NBC, inhibiting the growth of the gram-(+) and gram-(-) bacteria. The increased activity of NBC in relation to the clinical isolate of S. aureus compared to the museum strain is shown. The minimum inhibitory concentrations (MIC) of NBC for the studied test cultures of microorganisms ranged from 1.5 µg/ml to 3.5 µg/ml. The minimum bactericidal concentration (MBC) of NBC is in the range of 8–22 µg/ml. The results obtained indicate the prospects of the ‘green-synthesized’ product for medicine and veterinary medicine due to the combination of an environmentally safe synthesis method, prolonged mild action and low toxicity, as well as a wide spectrum of antimicrobial activity of NBC.

Key words: nanobiocomposite, silver nanoparticles, antimicrobial action, respiratory activity, lactobacilli, zeta-potential.

https://doi.org/10.15407/nnn.21.03.643

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