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T. O. Veklich, O. A. Shkrabak, Iu. V. Nikonishina, R. V. Rodik, V. I. Kalchenko, S. O. Kosterin
«Calix[4]arene C-90 as a Promising Supramolecular Compound to Regulate the Activity of Plasma Membrane Ca2+, Mg2+-ATPase of Smooth Muscle Cells»
373–380 (2017)

PACS numbers: 81.16.Fg, 82.39.Jn, 87.16.dp, 87.16.dr, 87.16.Tb, 87.19.Ff, 87.64.kv

The aim is to reveal the biochemical and physicochemical regularities of сalix[4]arene C-90 and its analogues’ effects on the activity of plasma membrane (PM) Ca2+, Mg2+-ATPase of myometrium, and functional response of the smooth muscle cells in the presence of calix[4]arene C-90. For this purpose, we use methods of enzymology, dynamic light scattering, and kinetic analysis. We show that the inhibitory effect of calix[4]arene C-90 on the activity of PM Ca2+, Mg2+-ATPase is caused by cooperative influence of four trifluoromethyl(phenylsulfonylimino)methylamino groups spatially oriented on calixarene platform. Calix[4]arene C-90 within the concentration range 1–50 mM do not influence on the coefficients of PM Ca2+, Mg2+-ATPase activation by ions of Ca, Mg and ATP, while mentioned compound dose-dependently decreases the maximum rate of enzymatic hydrolysis of ATP. Therefore, calix[4]arene C-90 is a complete non-competitive inhibitor of PM Ca2+, Mg2+-ATPase. We also show that calix[4]arene C-90, like uterotonik oxytocin, reduces the effective hydrodynamic diameter of the smooth muscle cells indicating a contraction of myocytes. Obtained results may be useful for the future studies aimed at using of calixarenes as potential pharmacological compounds for modulation of the uterine contractile function during the pathology of contractile activity.

Key words: Ca2+, Mg2+-ATPase, plasma membrane, myometrium, calix[4]arene.

https://doi.org/10.15407/nnn.15.02.0373

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