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В. Р. Эстрела-Льопис, А. В. Чевычалова, Е. М. Фесенко, Г. И. Довбешко, П. М. Литвин
«Гетерокоагуляция модифицированных полисахаридами и функционализированных фолатами наночастиц платины с раковыми клетками»
PACS numbers: 81.07.Pr, 82.45.Tv, 83.80.Lz, 87.15.-v, 87.19.xj, 87.64.-t, 87.85.Rs
The method for synthesis of targeted platinum nanoparticles (PtPS–F) functionalized by folate and modified by polysaccharides is developed, and the effect of these polysaccharides on tumour cells of different carcinogenesis is studied. Optimal conditions for the PtPS–F synthesis are revealed, based on spectral and electrosurface studies of formation of the adsorption layer of polysaccharides and folate on the surface of nanoparticles. The optimum concentration of reactants and the conditions (time, temperature, sequence of mixing) for the synthesis of the PtPS–F nanocomposites are established. The toxic effect of nanocomposites on the cancer cells and its selectivity in comparison with healthy cells are determined by means of investigation of heterocoagulation and kinetics of cytotoxic effect. The methods of atomic force, electron and optical microscopies as well as IR-spectroscopy are used.